Entanglement of polymer chains in hypertonic medium enhances the delivery of DNA and other biomacromolecules into cells
Aneta Karpińska, Alicja Zgorzelska, Karina Kwapiszewska, Robert Hołyst
Most experimental procedures applied in modern biology involve cargo delivering into cells. One of the ways to cargo introduction is osmotic-mediated intracellular vesicle swelling. However, its widespread use was hindered due to cargo size (<10 nm) and cell-type-related restrictions. We addressed the issue of the composition of colloidal loading solution to enhance the efficiency of cellular delivery.